Comparison of the chondrogenic differentiation potentials of hTMSCs cultured in serum-free medium versus those of hTMSCs cultured in serum-containing medium.
<p>Under chondrogenic conditions (A), visual assessment revealed similar levels of toluidine blue staining (x 400) in hTMSCs cultured under serum-free medium (left) and hTMSCs cultured under serum-containing medium (right). However, RT-PCR analysis of type II collagen and aggrecan mRNA expression in hTMSCs cultured under serum-free medium showed consistently increased expression, but much lower expression of chondrogenic differentiation markers compared with the levels in hTMSCs cultured under serum-containing medium.</p
