<p>(A) Ultrastructural studies showed regular foot processes (FP) in healthy control animals on the opposite side of endothelial cells (En) lining the capillary lumen (L). (B) Irregularly shaped or fused FPs in <i>Nphs2</i><sup><i>R140Q/-</i></sup> mice one week after induction (arrow). (C and D) Progression of focal changes to global fusion of FPs in <i>Nphs2</i><sup><i>R140Q/-</i></sup> animals over time (Magnification, X10000). 3D modelling of glomerular structure showed no GBM denudation in <i>Nphs2</i><sup><i>R140Q/-</i></sup> animal (F) compared to controls (E). Blue colour represents GBM, pink and green represent FPs of adjacent podocytes. Severely affected FP number and organization in <i>Nphs2</i><sup><i>R140Q/-</i></sup> animals (G). GBM thickening in <i>Nphs2</i><sup><i>R140Q/-</i></sup> animals (H) (G and H: analysis is based on 3 animals per group). ** p<0.01, **** p<0.0001.</p
