Additional file 5: Table S1. Clinicopathological characteristics of cervical carcinoma patient samples

Bing Zhang (129)

Guosheng Tan (4533616)

Heping Li (459150)

Huiru Tang (115310)

Liangshuai Liu (3588338)

Shiqiu Xiong (238109)

Wei Chen (23863)

Yan Wang (15435)

Verhoustraeten, Joël (1954-....). Photographe

Gallica, Bibliotheque Numerique

[Barco de Arena, par la Compagnie Leandre et Claire. Chalon dans la Rue. 2008 / photographies de Joël Verhoustraeten]

Abstract Background The aim of this study was to determine whether brain abundant membrane attached signal protein 1 (BASP1) is a valuable prognostic biomarker for cervical cancer and whether BASP1 regulates the progression of cervical cancer. Methods Quantitative real-time PCR, western blotting, and immunohistochemistry were used to determined BASP1 levels. Statistical analyses were used to examine whether BASP1 was a prognostic factor for patients with cervical cancer. The MTT assay, colony formation assay, cell cycle assay, anchorage-independent growth assay, and a tumor xenograft model were used to determine the role of BASP1 in the proliferation and tumorigenicity of cervical cancer. Results Brain abundant membrane attached signal protein 1 was upregulated in cervical cancer tissues and cells, and BASP1 expression levels were higher in patients that had died during follow-up compared with those that survived. There was a positive correlation between BASP1 expression and clinical stage (p < 0.001), T classification (p < 0.001), N classification (p < 0.05), and survival or mortality (p < 0.05). Patients with higher BASP1 expression had a shorter overall survival time. Cox regression analysis shown BSAP1 was an unfavorable prognostic factor for patients with cervical cancer. Overexpression of BASP1 promoted the proliferation of cervical cancer and its colony formation ability, accelerated cell cycle progression, and enhanced tumorgenicity. BASP1 knockdown inhibited the proliferation of cervical cancer and its colony formation ability, suppressed cell cycle progression, and decreased tumorgenicity. Conclusions The results showed that BASP1 not only is a novel prognostic factor for patients with cervical cancer, but also promotes the proliferation and tumorigenicity of cervical cancer

Huiru Tang

Yan Wang

Bing Zhang

Shiqiu Xiong

Liangshuai Liu

Wei Chen

Guosheng Tan

Heping Li

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Cancer Cell International

High brain acid soluble protein 1(BASP1) is a poor prognostic factor for cervical cancer and promotes tumor growth

Background: The aim of this study was to determine whether brain abundant membrane attached signal protein 1 (BASP1) is a valuable prognostic biomarker for cervical cancer and whether BASP1 regulates the progression of cervical cancer.   Methods: Quantitative real-time PCR, western blotting, and immunohistochemistry were used to determined BASP1 levels. Statistical analyses were used to examine whether BASP1 was a prognostic factor for patients with cervical cancer. The MTT assay, colony formation assay, cell cycle assay, anchorage-independent growth assay, and a tumor xenograft model were used to determine the role of BASP1 in the proliferation and tumorigenicity of cervical cancer.   Results: Brain abundant membrane attached signal protein 1 was upregulated in cervical cancer tissues and cells, and BASP1 expression levels were higher in patients that had died during follow-up compared with those that survived. There was a positive correlation between BASP1 expression and clinical stage (p &lt; 0.001), T classification (p &lt; 0.001), N classification (p &lt; 0.05), and survival or mortality (p &lt; 0.05). Patients with higher BASP1 expression had a shorter overall survival time. Cox regression analysis shown BSAP1 was an unfavorable prognostic factor for patients with cervical cancer. Overexpression of BASP1 promoted the proliferation of cervical cancer and its colony formation ability, accelerated cell cycle progression, and enhanced tumorgenicity. BASP1 knockdown inhibited the proliferation of cervical cancer and its colony formation ability, suppressed cell cycle progression, and decreased tumorgenicity.   Conclusions: The results showed that BASP1 not only is a novel prognostic factor for patients with cervical cancer, but also promotes the proliferation and tumorigenicity of cervical cancer.National Natural Science Foundation of China [30600156]; Natural Science   Foundation of Guangdong Province [2014A030313090, 2014A030313190];   Science and Technology Project Foundation of Guangzhou City   [1515000250]; Shenzhen Science and Technology Project (Basic Research)   [JCYJ20140415162338852]SCI(E)ARTICLE1

Tang, Huiru

Wang, Yan

Zhang, Bing

Xiong, Shiqiu

Liu, Liangshuai

Chen, Wei

Tan, Guosheng

Li, Heping

Institutional Repository of Peking University

High brain acid soluble protein 1(BASP1) is a poor prognostic factor for   cervical cancer and promotes tumor growth

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[Barco de Arena, par la Compagnie Leandre et Claire. Chalon dans la Rue. 2008 / photographies de Joël Verhoustraeten]

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Gallica, Bibliotheque Numerique

Directory of Open Access Journals

Institutional Repository of Peking University

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