<p>Assay controls were tested on TGA coated plates, with and without HRP competitor and mean absorbance was measured by OD. PC showed high initial OD and inhibition of ~80% with the addition of HRP, indicating recognition of plant glycans. NC showed high OD, both with and without HRP, indicating recognition of protein backbone. Normal serum pool (NSP) represented serum background levels with low OD, both with and without HRP, indicating low reactivity to TGA. Results, presented as a box plot graph, include data from 3 independent runs, showing individual measurements together with mean.</p