<i>In vitro</i> HIV infection does not affect RORC, RNA levels in blood-derived Th17 cells.

Abstract

<p>Blood-derived Th17 cells were infected with HIV for 24 hours. Input virus was washed out and cells were stimulated with anti-CD3 and anti-CD28 mAbs for 3 days. mRNA was isolated and quantified by real-time qPCR for RORC genes. Relative mRNA levels were calculated using the ΔΔCt method, normalized to 18S rRNA. Results are expressed as fold change relative to unstimulated, uninfected cells. n = 9.</p

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