In another independent assessment regarding the most abundant organism in the
vitreous samples we employed targeted PCR assays to detect the two most
frequently identified bacteria, <i>Staphylococcus
epidermidis</i> and <i>Enterococcus faecalis</i>.
For the detection of <i>S. epidermidis</i>
the species-specific primers SE705-1 (5’-ATC AAA AAG TTG GCG
AAC CTT TTC A-3’) and
SE705-2 (5’-CAA AAG AGC GTG GAG
AAA AGT ATC A-3’) were
used (Martineau <i>et al.</i> 1996).
For the detection of <i>E. faecalis</i> the
species-specific primers <i>ddl</i>-E1 (5’-ATC
AAG TAC AGT TAG TCT-3’)
and <i>ddl</i>-E2 (5’-ACG
ATT CAA AGC TAA CTG-3’) were
used (Dutka-Malen <i>et al</i>., 1995).<br><br><p><b>Reference</b></p>
<p><b>Kirstahler P,
Bjerrum SS, Friis-Møller A, </b><b>la Cour M, Aarestrup FM, Westh H., and Pamp SJ. (2018) </b>Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid.<b> </b><b>Scientific
Reports</b>, doi:10.1038/s41598-018-22416-4.</p
