Additional file 5: Figure S3. of AFEAP cloning: a precise and efficient method for large DNA sequence assembly

Abstract

Sequencing validation of plasmid sizes characterization. Five join sites are S1, S2, S3, S4, and S5. (a) 11.5 kb plasmid; (b) 19.6 kb plasmid; (c) 28 kb plasmid; (d) 34.6 kb plasmid. The overhang sequences were shown. (DOCX 3815 kb