[<i>SWI</i>⁺] cells have lower fitness and do not survive dry conditions.

Abstract

<p>(A) The survival of [<i>SWI</i>⁺] (red) and [<i>swi</i>^<i>−</i>] (blue) cells from overnight cultures (no stress), 6-day–starved cultures (starvation), and antifungal-treated overnight cultures (amphotericin B and caspofungin) were measured using a viability stain and flow cytometry. Error bars indicate standard deviation (<i>n</i> = 3). All numerical data in this figure and the flow cytometry gating strategy are available from the Dryad Digital Repository: <a href="https://doi.org/10.5061/dryad.d5r16" target="_blank">http://dx.doi.org/10.5061/dryad.d5r16</a>. (B) Growth comparison of [<i>swi</i>^<i>−</i>] cells and [<i>SWI</i>⁺] cells in media buffered at pH 7.5. Cell density was measured by absorbance every 15 minutes. (C) Survival of [<i>SWI</i>⁺] and [<i>swi</i>^<i>−</i>] cultures after removal of liquid media followed by drying under blowing air for 24 hours. The survival of [<i>SWI</i>⁺] was below the detection limit using viability stain and flow cytometry (1 in 10,000 cells). Error bars indicate standard deviation (<i>n</i> = 3). (D) Recovery of dried [<i>SWI</i>⁺] and [<i>swi</i>^<i>−</i>] strains in liquid culture in microtiter plates. After rehydration of dried cells into the original volume of the liquid culture, a 25-fold dilution into fresh media was made in microtiter wells. Growth after four days is depicted.</p