<p>(A) Representative I-V plot of NaChBac obtained from single-channel recording. Data points are summarized from single-channel event analysis. If no data point displayed, it means that not enough events were found in that particular voltage step. While L1 (red square) opened in both positive and negative step voltage, L3 (green triangle) and L2 (blue diamond) open less frequently at negative step voltage. (B) Distribution of the open probability of the three sub-states at different voltage based on the same experiment of (A). Data were analyzed by all-point current histogram. (C) The total open probability of NaChBac depends on the holding voltage.</p
