Syndecan-1 protein level following transfection with the full-length syndecan-1 (FLs1), nuclear localization signal deleted syndecan-1 (NLSdel) and empty vector control (EV). (a) Representative histogram of syndecan-1 protein level detected by Fluorescence Activated Cell Sorting (FACS) analysis. Dotted line represents the IgG control, green line corresponds to empty vector and the blue and red line to the full-length syndecan-1 (FLs1) and nuclear localization signal deleted syndecan-1 (NLSdel), respectively. (b) Quantitative syndecan-1 protein level by FACS analysis corresponding to three independent experiments. Error bars represent standard error of the mean (SEM). * denotes statistically significant differences. (c) Relative syndecan-1 levels measured by western blotting, using actin as loading control. (JPEG 175 kb
