Multilocus Sequence Analysis of 14 genes, recA, gltA, glnA, ctrA, ftsA, ftsZ1, ftsZ2, gyrB, dnaK, pnp, rpoB, thrC, atpD and gap in E. meliloti strains FSM-MA, Sm1021, Su47, Rm41, AK58, AK83, SM11, GR4, BO21CC and BL225C, E. arboris strain LMG14919, E. medicae strain WSM419 and E. fredii strains USDA257, NGR234 and HH103. The concatenated gene sequences (total 23,220 bp) were aligned by ClustalW and a maximum likelihood tree was inferred from the aligned sequences using MEGA ver. 6.0.6 software (Tamura et al., 2007). The tree was estimated using the Tamura-Nei substitution model (Tamura and Nei, 1993). Bootstrap tests were performed with 1000 replications. The inset shows the topology of the maximum likelihood tree. Tamura K, Nei M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10: 512–526. Tamura K, Dudley J, Nei M, Kumar S. (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596–1599. (TIFF 14278 kb
