<p>A new tirucallane-type triterpenoid igniarine (<b>1</b>), and four known compounds meshimakobnol A (<b>2</b>), meshimakobnol B (<b>3</b>), ergosterol (<b>4</b>) and ergosterol peroxide (<b>5</b>), were purified from the methanol extracts of the fruiting bodies of <i>Phellinus igniarius</i> (DC. ex Fr.) Quél. The structure of <b>1</b> was elucidated using a combination of 1D and 2D NMR techniques and HR-ESI-MS analyses. In addition, the isolated compounds were examined for their cytotoxicity against several tumour cell lines and part of the tested compounds demonstrated weak cytotoxicity.</p
