Aki Fujiwara-Igarashi (4684408)

Daisuke Hasegawa (239088)

Michio Fujita (502777)

Shunta Mizoguchi (4684411)

Takayuki Kuwabara (1841191)

Yoshihiko Yu (4684414)

Yuji Hamamoto (3221127)

BMC Veterinary Research

Multiple alignment of LGI1 amino acid sequences from Felis catus and other species. Multiple alignment was constructed using DNAMAN 9.122. The black, red, and light blue shading represent 100% conserved, less-conserved (âĽ75%), and non-conserved (âĽ50%) amino acids, respectively (JPEG 14409 kb

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Additional file 6 of Molecular cloning and characterization of the family of feline leucine-rich glioma-inactivated (LGI) genes, and mutational analysis in familial spontaneous epileptic cats

Exonic structures of the coding sequences of the LGI family genes from Felis catus and other species. (aâd) show comparison of exonic structures between species for the LGI1âLGI4 genes, respectively. Amino acid sequences and genomic sequences are provided in Additional file 5. Colored blocks represent exons. Gray blocks represent introns. Numbers over the colored blocks indicate base pairs that construct exons (TIFF 15627 kb

Additional file 4: of Molecular cloning and characterization of the family of feline leucine-rich glioma-inactivated (LGI) genes, and mutational analysis in familial spontaneous epileptic cats

Allelic and genotypic distribution of synonymous and intronic polymorphisms other than non-synonymous mutation found in LGI1â4 genes in familial spontaneous epileptic cats (FPSCs) and controls (DOCX 86 kb

Additional file 11 of Molecular cloning and characterization of the family of feline leucine-rich glioma-inactivated (LGI) genes, and mutational analysis in familial spontaneous epileptic cats

Abstract Background Leucine-rich glioma-inactivated (LGI) proteins play a critical role in synaptic transmission. Dysfunction of these genes and encoded proteins is associated with neurological disorders such as genetic epilepsy or autoimmune limbic encephalitis in animals and human. Familial spontaneous epileptic cats (FSECs) are the only feline strain and animal model of familial temporal lobe epilepsy. The seizure semiology of FSECs comprises recurrent limbic seizures with or without evolution into generalized epileptic seizures, while cats with antibodies against voltage-gated potassium channel complexed/LGI1 show limbic encephalitis and recurrent limbic seizures. However, it remains unclear whether the genetics underlying FSECs are associated with LGI family genes. In the present study, we cloned and characterized the feline LGI1–4 genes and examined their association with FSECs. Conventional PCR techniques were performed for cloning and mutational analysis. Characterization was predicted using bioinformatics software. Results The cDNAs of feline LGI1–4 contained 1674-bp, 1650-bp, 1647-bp, and 1617-bp open reading frames, respectively, and encoded proteins comprising 557, 549, 548, and 538 amino acid residues, respectively. The feline LGI1–4 putative protein sequences showed high homology with Homo sapiens, Canis familiaris, Bos taurus, Sus scrofa, and Equus caballus (92%–100%). Mutational analysis in 8 FSECs and 8 controls for LGI family genes revealed 3 non-synonymous and 14 synonymous single nucleotide polymorphisms in the coding region. Only one non-synonymous single nucleotide polymorphism in LGI4 was found in 3 out of 8 FSECs. Using three separate computational tools, this mutation was not predicted to be disease causing. No co-segregation of the disease was found with any variant. Conclusions We cloned the cDNAs of the four feline LGI genes, analyzed the amino acid sequences, and revealed that epilepsy in FSEC is not a monogenic disorder associated with LGI genes

Yoshihiko Yu

Daisuke Hasegawa

Aki Fujiwara-Igarashi

Yuji Hamamoto

Shunta Mizoguchi

Takayuki Kuwabara

Michio Fujita

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Molecular cloning and characterization of the family of feline leucine-rich glioma-inactivated (LGI) genes, and mutational analysis in familial spontaneous epileptic cats

http://catalogue.bnf.fr/ark:/12148/cb303310373