Additional file 3: Figure S1. of In vivo imaging of epithelial wound healing in the cnidarian Clytia hemisphaerica demonstrates early evolution of purse string and cell crawling closure mechanisms
Labeling index of cells in the exumbrella. EdU incorporation showed that in the first 3–4 days after release there is very little, if any, division in epithelial cells in the exumbrella. In contrast, at 7 days the percentage of cells dividing within a 24 h period is >40%. By two and three weeks, the number of dividing cells per 24 h is greatly reduced, and declines further as animals age. Therefore, in the 2–3 week old animals used in wounding assays there is little epithelial cell division in the exumbrella. Animals were labelled for 24 h with EdU, and then fixed and stained with Hoescht stain. Values are the percentage of Hoechst stained cells that also showed EdU labeling. 3–5 animals were examined at each time point. Error bars = s.e.m. (PPTX 53 kb
