16 mars 19361936/03/16 (A16,N5535)-1936/03/16

Alexander Ishov (3479018)

Serena Giovinazzi (47441)

Viacheslav Morozov (4716216)

Additional file 4: Fig. S4. Top: SUMO depletion efficiency (relevant to Fig. 4). HEp2 cells expressing FLAG-HA-CENP-B (pOZ-CENP-B) were transfected with scrambled siRNA (CTL), SUMO-1 or -2 siRNA and treated with MG132 for 4 h. Cell lysates were analyzed by Western blot and probed with SUMO-1 (left) or SUMO-2 (right) antibodies. Control: un-transfected HEp2 cells (pOZ). Both SUMO siRNA reduced levels of corresponding conjugates. Bottom: SUMO depletion does not change levels of endogenous Daxx (relevant to Fig. 3c). Conditions as above; chromatin-associated fractions were analyzed by Western blot and probed with Daxx antibodies. *: unspecific band. Control: un-transfected HEp2 cells (pOZ). SUMO-1 or -2 depletion does not affect levels of Daxx

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MOESM4 of CENP-B protects centromere chromatin integrity by facilitating histone deposition via the H3.3-specific chaperone Daxx

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Gallica, Bibliotheque Numerique

Le Peuple : organe quotidien du syndicalisme

http://gallica.bnf.fr/ark:/12148/cb34348990g/date

Le Peuple : organe quotidien du syndicalisme

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Gallica, Bibliotheque Numerique