Presynaptic requirement for Gef26 in synaptic growth regulation and characterization of satellite boutons. a Confocal images of anti-HRP-labeled NMJ 6/7 in C155-GAL4/+, C155-GAL4/+; UAS-gef26 RNAi /+, BG57-GAL4/+, and BG57-GAL4/UAS-gef26 RNAi third-instar larvae. Scale bar, 20 μm. b Quantification of total bouton number and satellite bouton number. c-e Confocal images of NMJ 6/7 stained with anti-HRP and anti-NC82 (c), anti-CSP (d), or anti-Dlg (e) for wild-type and gef26 6 /Df third-instar larvae. f Confocal images of NMJ 6/7 stained with anti-NC82 and anti-GluRIIC in wild-type and gef26 6 /Df third-instar larvae. The number of NMJs analyzed is indicated in each bar. Data are expressed as mean ± SEM. *P < 0.001. (TIFF 20874 kb
