Correlative light and electron microscopy of cryostat sections of control iPSC optic cup using dual fluorescence microscopy (FM) and nano-gold rhodopsin labelling.
<p>(A) Diagram outlining the differentiation of inducible pluripotent stem cells (iPSCs) into optic cups with photoreceptors. (B) Confocal FM overlay on top of differential interference contrast (DIC) image (C) FM overlay on top of electron microscopy (EM) image. (D) EM with (E) a high magnification region showing concentrated rhodopsin localisation in the photoreceptor OS region of the optic cups. The connecting cilia in (D) is indicated by CC. Scale bar = (B & C) 100um, (D) 2um and (E) 200nm.</p
