Effects of diet manipulation and 1,25 (OH)<sub>2</sub> vitamin D supplementation on NF-κB activation (panel A) and TNF-alpha levels (panel B) in the mouse gastrocnemious.
<p>(A) Representative western blotting analysis for the expression of NF-κB p65 subunit. Protein expression was analyzed by Western blot on cytosol and nucleus homogenates of gastrocnemious from animals fed a control diet or HFHS diet with or without vitamin D supplementation (7 μg/kg, 3 times per week). Densitometric analysis of the bands is expressed as relative optical density (O.D.) corrected for the GADPH (cytosol) or histone (nucleus) contents, and normalized using the related control band. NF-κB p65 subunit translocation was expressed as nucleus/cytosol ratio normalized using the related control band. (B) TNF-alpha levels were measured by ELISA in the mouse gastrocnemious homogenates. The data are expressed by medians and interquartile range) of 5–6 randomly selected animals per group. Statistical analysis was performed with Kruskal–Wallis test with Dunn’s post hoc test. *p<0.05 <i>vs</i> Control; #p<0.05 <i>vs</i> HFHS.</p
