Additional file 5: Figure S4. of Gibson Deletion: a novel application of isothermal in vitro recombination

Abstract

Sequences of the incorrect Gibson Deletion of 200 nt related to Fig. 5. Sequences of the DNA flanking the RFP cassette (red box) cloned in pUC19 using Gibson Deletion. The RFP cassette was PCR amplified using primers with homology arms aimed to delete 200 nts (100 nucleotides from each side of a KpnI cut) from the pUC19 plasmids (Fig. 5). All the clones picked and sequences had incorrect assembly and are presented here together with the predicted sequence on top (correct). Underlined sequences are part of the RFP cassette; red sequences are sequences that are deleted after assembly using Gibson Deletion; sequences in bold are insertions obtained after assembly. (PDF 1358 kb