Jiapan Liu (4787340)

Jiazuan Ni (425336)

Jieqiong Li (327149)

Jing Tian (335437)

Jingxin Zheng (4787337)

Lifang Chen (522518)

Qiong Liu (299371)

Yujuan Wang (436153)

Nutrition & Metabolism

Gallica, Bibliotheque Numerique

La Toque blanche : organe professionnel hebdomadaire des chefs de cuisine de France et de l'étranger

BACKGROUND: Selenoprotein F (SELENOF, was named as 15-kDa selenoprotein) has been reported to play important roles in oxidative stress, endoplasmic reticulum (ER) stress and carcinogenesis. However, the biological function of SELENOF is still unclear. METHODS: A yeast two-hybrid system was used to screen the interactive protein of SELENOF in a human fetal brain cDNA library. The interaction between SELENOF and interactive protein was validated by fluorescence resonance energy transfer (FRET), co-immunoprecipitation (co-IP) and pull-down assays. The production of retinol was detected by high performance liquid chromatograph (HPLC). RESULTS: Retinol dehydrogenase 11 (RDH11) was found to interact with SELENOF. RDH11 is an enzyme for the reduction of all-trans-retinaldehyde to all-trans-retinol (vitamin A). The production of retinol was decreased by SELENOF overexpression, resulting in more retinaldehyde. CONCLUSIONS: SELENOF interacts with RDH11 and blocks its enzyme activity to reduce all-trans-retinaldehyde

Tian, Jing

Liu, Jiapan

Li, Jieqiong

Zheng, Jingxin

Chen, Lifang

Wang, Yujuan

Liu, Qiong

Ni, Jiazuan

Hochschulbibliothekszentrum des Landes Nordrhein-Westfalen (hbz)

The interaction of selenoprotein F (SELENOF) with retinol dehydrogenase 11 (RDH11) implied a role of SELENOF in vitamin A metabolism

The reductase activity of SELENOFâ toward all-trans-retinaldehyde. The plasmid SELENOFâ-PET28b was transfected into E.coli (BL21) and recombinant SelenoFâ was induced and then the cell lysates were used to detect the enzyme activity to reduce all-trans-retinaldehyde (column A); Empty vector PET28b was transfeced into E.coli (BL21) and used as the negative control (column B). (DOCX 53 kb

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Additional file 3: Figure S3. of The interaction of selenoprotein F (SELENOF) with retinol dehydrogenase 11 (RDH11) implied a role of SELENOF in vitamin A metabolism

Jing Tian

Jiapan Liu

Jieqiong Li

Jingxin Zheng

Lifang Chen

Yujuan Wang

Qiong Liu

Jiazuan Ni

Crossref

Abstract Background Selenoprotein F (SELENOF, was named as 15-kDa selenoprotein) has been reported to play important roles in oxidative stress, endoplasmic reticulum (ER) stress and carcinogenesis. However, the biological function of SELENOF is still unclear. Methods A yeast two-hybrid system was used to screen the interactive protein of SELENOF in a human fetal brain cDNA library. The interaction between SELENOF and interactive protein was validated by fluorescence resonance energy transfer (FRET), co-immunoprecipitation (co-IP) and pull-down assays. The production of retinol was detected by high performance liquid chromatograph (HPLC). Results Retinol dehydrogenase 11 (RDH11) was found to interact with SELENOF. RDH11 is an enzyme for the reduction of all-trans-retinaldehyde to all-trans-retinol (vitamin A). The production of retinol was decreased by SELENOF overexpression, resulting in more retinaldehyde. Conclusions SELENOF interacts with RDH11 and blocks its enzyme activity to reduce all-trans-retinaldehyde

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Expression level of recombinant SELENOF-HA or RDH11-YFP. The overexpressed SELENOF-HA or RDHA11-YFP was detected by anti-HA antibody (A) or anti-GFP antibody (B). In part A, lane 1 shows HEK293T cells co-transfected with SELENOF-HA-SelExpress1 and RDH11-YFP; lane 2 shows HEK293T cells co-transfected with plasmids SELENOF-HA-SelExpress1 and EYFP empty vector. In part B, lane 1 shows HEK293T cells co-transfected with plasmids SELENOF-HA-SelExpress1 and RDH11-YFP; lane 2 shows HEK293T cells co-transfected with plasmids HA-SelExpress1 empty vector and RDH11-YFP. (DOCX 84 kb

Additional file 2: Figure S2. of The interaction of selenoprotein F (SELENOF) with retinol dehydrogenase 11 (RDH11) implied a role of SELENOF in vitamin A metabolism

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La Toque blanche : organe professionnel hebdomadaire des chefs de cuisine de France et de l'étranger

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Gallica, Bibliotheque Numerique

Hochschulbibliothekszentrum des Landes Nordrhein-Westfalen (hbz)

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