Schematic representation of the construction of the recombinant spores.

Abstract

<p>The <i>cotC</i>::<i>ompC</i> gene fusion and <i>cat</i> (chloramphenicol-resistance gene) gene carried by plasmid pDG364-<i>cotC</i>-<i>ompC</i> were integrated into the <i>amyE</i> gene locus of <i>B</i>. <i>subtilis</i> 168 chromosome by double cross-over recombination events. Arrows indicate direction of transcription.</p