Primers used in the genetic engineering. Table S2. The gradient variation ratio of phases A:B in HPLC analysis of L-Phe concentration. Table S3. Primers used in the Real-time PCR analysis. Figure S1. Fermentation consequence of the tyrR knocked out strain. The gray column means OD600 and the black column means L-Phe titer. Figure S2. Sequence alignment results of the TyrRwt and TyrRmut. This result was obtained by DNAMAN software. Figure S3. Overexpression of the precursorsâ synthesis encoded genes. The gray column means OD600 and the black column means L-Phe titer. Figure S4. Relative strength of five promoters that were used to overexpress AroD in xllp08. (DOCX 582Â kb
