In this study, the effects of Mannich bases containing bis-1,2,4-triazole on the levels of in vivo malondialdehyde (MDA) and antioxidant
vitamins (A, E, C) were examined in serum, livers and kidneys of rats. DA and vitamin (A, E, C) levels were determined by high
performance liquid chromatography (HPLC). Antioxidant effect was investigated by determining the MDA levels in Saccharomyces cerevisiae
cells as in vitro. Furthermore, the antitumor effects of compounds were investigated against MCF-7 human breast cancer cells. Interrelations of
results among control and compound groups were evaluated using SPSS statistical software package. As a result, some of the compounds showed
effective biological activity when compared to control conditions. The test compounds used in this study may be effective for utilization in the
selection and design of model compounds for further studies