Noninvasive metabolic assessment of human donor livers - Prognostic value of P-31-magnetic resonance spectroscopy for early graft function

Abstract

Background. The purpose of this study was to investigate whether phosphorus-31 magnetic resonance spectroscopy (P-31-MRS) of the isolated donor liver can serve as a viability indicator with prognostic value for transplantation outcome. Methods. Forty human donor livers preserved with University of Wisconsin solution were studied shortly before transplantation. The respective spectral peak areas of the isolated donor liver were correlated with the amount of hepatocellular graft damage and liver metabolic function shortly after implantation. Results. The individual phosphomonoesters, inorganic phosphate, phosphodiesters, and nicotine adenine dinucleotide peaks were not prognostic for postoperative hepatocellular damage or liver metabolic capacity. The presence of adenosine triphosphate, however, predicts a significantly better metabolic capacity to eliminate bilirubin, to synthesize fibrinogen and antithrombin III, and to maintain a better prothrombin time after transplantation. Furthermore, this study is probably the first P-31-MRS demonstration in the human liver of phosphocreatine, Conclusions. In the clinical setting described, metabolic assessment using P-31-MRS did not result in a reliable noninvasive test to predict primary graft dysfunction. Study of the role of phosphocreatine in liver metabolism during cold storage is needed