The morphological and functional property in the skeletal muscle is associated with intracellular Ca2+([Ca2+]i)homeostasis. In this thesis,[Ca2+]i homeostatic control mechanism was investigated by in vivo bioimaging on animal model of diabetes mellitus to associate with fragility of the skeletal muscle. As a result,the diabetic animal model found the phenomenon that Ca2+ readily accumulated in cytoplasm by muscle contractions. This study developed Ca2+ injection technique to muscle fiber to clarify mechanism of the dysfunction of [Ca2+]i homeostasis. These studies model demonstrated that the Ca2+ release-uptake system was impaired in the diabetes animals. In addition,this study confirmed that the depression of [Ca2+]i homeostasis occurred in fast and slow muscle in diabetes. This study evidenced that fragility of diabetic skeletal muscle was associated with depression of the [Ca2+]i homeostasis by an in vivo bioimaging.電気通信大学201
