Benito-Lopez, Fernando

Czugala, Monika

Diamond, Dermot

DCU Online Research Access Service

ATWARMAdvanced	  Technologies	  for	  Water	  Resource	  ManagementMarie	  Curie	  Ini+al	  Training	  NetworkNext	  Genera;on	  Autonomous	  Analy;cal	  Pla>orms	  for	  Remote	  Environmental	  MonitoringGenera&on	  of	  Fully	  Func&oning	  Biomime&c	  Analy&cal	  Pla5orms	  for	  Water	  QualityM.	  Czugala,	  F.	  Benito-­‐Lopez	  and	  D.	  Diamond	  CLARITY:	  Centre	  for	  Sensor	  Web	  Technologies,	  Na+onal	  Centre	  for	  Sensor	  Research,	  Dublin	  City	  University,	  Dublin	  9,	  IRELANDCentrifugal	  Microﬂuidics	  Analysis	  System	  (CMAS)	  Introduc&onConclusionsCD	  pla5ormNowadays,	   there	   is	   great	   interest	   in	   in-­‐situ	   monitoring	   allowing	   fast	   data	   acquisi7on	   for	  environmental	   analysis.	   The	   challenge	   is	  to	  develop	  autonomous	  devices	  that	  could	  be	   used	   in	  remote	   loca7ons,	  which	  would	  ul7mately	   enable	   dynamic	   monitoring.	  Typical	  analysis	   methods	  are	   very	   costly	   and	   7me	   consuming,	   therefore	   addressing	   the	   need	   for	   a	   simple	   and	   cost-­‐eﬀec7ve	   sensor	   is	   crucial.	   Here	   we	   present	   the	   design	   and	   the	   development	   of	   a	   portable	  wireless	   centrifugal	   microﬂuidic	   analysis	   system	   (CMAS)	   for	   a	   lab-­‐on-­‐a-­‐disc	   water	   quality	  monitoring.	  The	  micro-­‐ﬂuidic	  plaGorm	  enables	  in	  situ	  nitrite	  detec7on	  of	  real	  water	  samples.a)b)0.2 mg/L0.4 mg/L0.6 mg/L0.8 mg/L1.0 mg/L1.2 mg/L0.0 mg/La)!!23514! !!0 0.5 1 1.5 2 2.5 0 5 10 15 20 25 30 35 Normalised Absorbance [A.u.] Time [min] 0.2 mg/L 0.4 mg/L 0.6 mg/L 0.8 mg/L 1.0 mg/L 1.2 mg/L y = 1.6458x + 0.0736 R² = 0.99321 0 0.5 1 1.5 2 2.5 0 0.2 0.4 0.6 0.8 1 1.2 1.4 Normalised Absorbance [A.u.] Concentration [mg/L] Absorbance after 20 mins ResultsCareer	  developmentAcknowledgementsc)Fig.	   4.	   Kine7c	   study	   of	   the	   colour	   forma7on	  monitored	   at	   a	   wavelength	   λmax	   =	   540	  nm	   between	  NO2-­‐	  and	   Griess	   reagent	   (n	   =	   3)	   (leQ	   side)	   and	  absorbance	   versus	  nitrite	   Griess	   reagent	  complex	  concentra7on	  (right	  side)	  using	  a	  UV-­‐Vis	  spectrometer	  (a)	  and	  the	  CMAS	  system	  (b).a)b)Marie	  Curie	  Ini7al	  Training	  Network	  funded	  by	  the	  EC	  FP7	  People	  Programme.Science	  Founda7on	  Ireland	  under	  grant	  07/CE/I1147.•	  Lab-­‐on-­‐a-­‐Chip	  European	  Congress,	  28-­‐29	  March	  2012,	  Edinburgh,	  Scotland	  (POSTER)	  •	  6th	  Interna7onal	  Conference	   on	  Environmental	  Science	   and	  Technology	   2012,	  June	   25-­‐29,	  2012,	  Houston,	  USA	  (ORAL)	  •	  Sino-­‐European	  Symposium	  on	  Environment	  and	  Health,	  20-­‐25	  Aug	  2012,	  Galway,	  Ireland	  (ORAL)•	  The	   16th	   Interna7onal	   Conference	  on	  Miniaturized	  Systems	   for	  Chemistry	   and	   Life	   Sciences,	   28	  Oct	  -­‐	  1	  Nov,	  2012,	  Okinawa	  Short Courses•	  Workshop:	  “Sensing:	  Changing	  the	  way	  we	  live	  our	  lives”,	  21	  Nov	  2011,	  DCU,	  Ireland•	  “Stats	  and	  data	  processing	  course”,	  13-­‐14	  March	  2012,	  DCU,	  Dublin,	  Ireland•	  CMA	  Analy7cal	  Workshop	  2012,	  24-­‐26	  April,	  Trinity,	  Dublin,	  Ireland•	  Cambridge	  Cer7ﬁcate	  in	  Advanced	  English	  English	  Course	  (CAE),	  2012ConferencesFig.	   3.	   	  a)	  Scheme	   showing	   the	   assembly	  of	   the	   micro-­‐ﬂuidic	   CD,	  b)	   picture	   of	   the	   Lab-­‐on-­‐a-­‐Disc	  and	   channel	   consis7ng	   of	   three	   chambers	  and	   c)	   a	   zoom	   on	   a	   single	   chip	   consis7ng	   of	   three	  chambers.Fig.	   5.	  Water	  nitrite	   analysis	   using	   a	  bench-­‐top	  UV-­‐VIS	   spectrometer	  and	  the	  CMAS	  device	   and	   a	  map	  of	  the	  sampling	  places	  (n	  =	  3).The	   autonomous	   capabili7es	   of	   the	   CMAS	   unit,	   combined	   with	   the	   portability	   and	   wireless	  communica7on,	   provide	   the	   ﬂexibility	   needed	   for	   on-­‐site	   water	   analysis.	   Apart	  from	   the	   fact	   that	  acquisi7on	   parameters	   can	   be	   controlled	   remotely	   and	   adjusted	   according	   to	   individual	   needs,	  results	  can	  be	   downloaded	  in	  remote	   loca7ons	  and	  displayed	   in	   real	  7me.	   	  The	   system	  demonstrates	  the	  poten7al	  of	  a	  truly	  autonomous	  micro-­‐ﬂuidic	  plaGorm	  for	  aqua7c	  environmental	  analysis.	  Fig.	  1.	  	  Picture	  of	  the	  CMAS	  with	  a	  CD	  microﬂuidic	  in	  it	  (leQ),	  and	  the	  centrifugal	  microﬂuidic	  analysis	  system	  major	  components	  (right).Fig.	  2.	  Portable	  centrifugal	  microﬂuidic	  analysis	  system	  (leQ),	  and	  conﬁgura7on	  of	  the	  paired	  LEDs	  for	  the	  alignment	  of	  the	  CD	  and	  the	  colorimetric	  analysis.	  Wednesday 25 April 12

Generation of fully functioning biomimetic analytical platorms for water quality

Irish Universities

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Generation of fully functioning biomimetic analytical platorms for water quality

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Irish Universities

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