Healthy and active lifestyle has led in recent years to the rapid development of antimicrobial treatment. Such antimicrobial materials provide lasting freshness and a sense of security and well-being of consumers. At first glance it seems easy to achieve antimicrobial properties, but the persistence of such treatment is a bigger problem. Application of natural zeolite nanoparticles for antimicrobial protection has shown increased activity and synergism with some antimicrobial agents, e.g. azalide. On the other hand, azalides are not acceptable from dermatological or ethics view, because of resistance development. This paper deals with the selection of optimal antimicrobial treatment which will provide protection against Gram positive (Staphylococcus aureus) and Gram negative (Klebsiella pneumoniae) bacteria, as well as fungi (Candida albicans). For this purpose, 100 % raw and bleached cotton knitted fabric was treated by exhaustion with natural zeolite nanoparticles and different antimicrobial agents cationic surfactant, azalide and antiseptic. It is well known that the cationic surfactants significantly improve fabric hand, but the high concentrations of activated zeolite getting it worse. Therefore, the influence of these treatments on fabric hand (subjective and objective evaluated) was investigated
