Pósa, Annamária

English

Pósa Annamária

University of Szeged

Volume 59(1):91-101, 2015 Acta Biologica Szegediensis http://www2.sci.u-szeged.hu/ABS Detection of Mycobacterium tuberculosis complex ancient DNA in human skeletal remains Annamária Pósa1'2 'Department of Biological Anthropology, University of Szeged, Szeged, Hungary, 2Archaeogenetics Laboratory, Institute of Archaeology, Research Centre for the Humanities, Hungarian Academy of Sciences, Budapest, Hungary The aim of this research was to detect the presence of the causative agent of tuberculosis (TB). the Mycobacterium tuberculosis complex (MTBC) in ancient Hungarian human remains, in order to obtain a better understanding of the prevalence and pathology of this devastating disease throughout different historical time periods. We have analyzed skeletal remains dating from the Neolithic to the Late Medieval periods (including Bronze Age, Avar Age and Ár-pádian Age samples). All the skeletal series selected for investigation contained cases showing macromorphological skeletal alterations indicative of TB. Both classical/advanced stage bone lesions and atypical/early-stage symptoms were searched for. The bone material has been screened for the presence of MTBC aDNA both in morphologically positive and negative cases. The members of the MTBC contain a multicopy repetitive insertion sequence element called 1S6110. The presence of TB aDNA was assessed by applying a PCR-based assay targeting the MTBC IS6110 region. To increase the sensitivity of the assay, a nested PCR strategy was applied. Initially, conventional PCR was performed using primers IS6110V and IS6110R to generate a 123 base pair product. The examination was carried out in a special pre-PCR area with a stringent environment for the studies of aDNA including the use of protecting clothing, UV-light exposure of the equipment and bleach sterilization of the surfaces. The aDNA work has been carried out in the aDNA laboratory of the EURAC Institute for Mummies and the Iceman in Bolzano and the Archaeogenetics Laboratory of the Institute of Archaeology, Research Centre for the Humanities, Hungarian Academy of Sciences. Our preliminary biomolecular results indicate high prevalence of TB infection in the Late Medieval population of Bácsalmás-Oalmás (Pósa et al. 2013). Further analyses were carried out in this particular series, however, has drawn attention to the complementarity of biomolecular and macromorphological investigations; in addition to double-positive cases, morphologically negative/PCR positive and morphologically positive/PCR negative cases have also came to light in the course of the examinations (Pósa et al. 2015a). As for the sampling strategies, remnants of MTBC aDNA were found in skeletons from both sexes and all age groups. Corresponding to the scientific literature, we have sampled compact bone for DNA extraction in each case. However, we have also made several successful attempts to follow protocols of sampling teeth for MTBC aDNA extraction (Faerman et al. 1999; Nguyen-Hieu et al. 2011; Pósa et al. 2012). We also carried out a paleomicrobial study of the Alsónyék Neolithic series. Relatively high prevalence of TB infection was proved in the grave group no. 13 (Pósa et al. 2015b). The PCR analyses of some of the included series are still in progress, similarly to the ongoing spoligo-typing and sequencing of the previously extracted MTBC aDNA remains possibly providing more detailed information on the different MTBC pathogens. References Faerman M, Jankauskas R. Gorski A, Bercovier H, Greenblatt CL (1999) Detecting Mycobacterium tuberculosis DNA in medieval skeletal remains from Lithuania. In Pálfi Gy, Dutour O. Deák J, Hutás I eds.. Tuberculosis Past and Present. Golden Book Publisher Ltd. pp. 371-376. Nguyen-Hieu T, Aboudharam G, Drancourt M (2011) Mini Review: Dental Pulp as a Source for Paleomicrobiology. Bull Int Assoc Pa-rodont 5(l):48-54. Pósa A, Maixner F. Zink A, Lovász G, Molnár E, Bereczki Zs, Perrin P. Dutour O, Sola O, Pálfi Gy (2012) Ancient human tooth samples used for TB paleomicrobial research. Acta Biol Szeged 56(2): 125-131. Pósa A, Maixner F, Lovász G, Molnár E, Bereczki Zs, Zink A, Pálfi Gy (2013) Revision of tuberculous lesions in the Bácsalmás-Óalmás series preliminary morphological and biomolecular studies. Anthropol Anz 70(1):83-100. Pósa A, Maixner F, Sola Ch, Bereczki Zs, Molnár E, Masson M. Lovász G. Spekker O. Wicker E, Zink A, Perrin P. Dutour O, Zink A. Pálfi Gy (2015a) Tuberculosis infection in a late-medieval Hungarian population. Tuberculosis, DOI: http://dx.doi.Org/10.1016/j. tube.2015.02.010. Pósa A, Maixner F, Mende B G. Köhler K, Osztás A, Sola Ch. Dutour O. Masson M, Molnár E. Pálfi Gy, Zink A (2015b) Tubercu-losis in Late Neolithic-Early Copper Age human skeletal remains from Hungary. Tuberculosis. DOI: http://dx.doi.0rg/lO.lOl6/j. tube.2015.02.011. Supervisors: György Pálfi. Balázs Gusztáv Mende E-mail: posa.annamaria@gmail .com 97 

Detection of Mycobacterium tuberculosis complex ancient DNA in human skeletal remains

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Detection of Mycobacterium tuberculosis complex ancient DNA in human skeletal remains

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