We investigated the effect of exogenous NO on PSI photochemistry by measuring P700 absorbance changes at 810-870 nm in intact pea leaves treated with the NO donor GSNO. Our results indicate that NO increases PSI quantum efficiency and the pool size of electrons in the intersystem chain

Horváth, Ferenc

Wodala, Barnabás

English

Wodala Barnabás

Horváth Ferenc

University of Szeged

Volume 52(1):243-245, 2008Acta Biologica Szegediensishttp://www.sci.u-szeged.hu/ABSARTICLEDepartment of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged, HungaryThe effect of exogenous NO on PSI photochemistry in intact pea leavesBarnabás Wodala*, Ferenc HorváthABSTRACT                        We investigated the effect of exogenous NO on PSI photochemistry by measuring P700 absorbance changes at 810-870 nm in intact pea leaves treated with the NO donor GSNO. Our results indicate that NO increases PSI quantum efficiency and the pool size of electrons in the intersystem chain. Acta Biol Szeged 52(1):243-245 (2008)KEY WORDSPSIphotosynthetic electron transport P700 absorbanceNO effectGSNOMaterials and MethodsPlant material and experimental solutionsTable 1. PSI and electron pool size values calculated from P700 oxidation curves measured in pea leaf disks that were incubated for 70 min under 150 µmol m-2 s-1 white light in distilled water (Control) or solutions of GSNO and Hb, as indicated. GSNO and Hb were used in concentrations of 1 mM and 4 mg mL-1, respec-tively. Values represent means ± SD (n = 8).PSI Pool size of electrons in the intersystem chainPool size of electrons from stromal donorsControl 0.41 ± 0.01 13.16 ± 2.75 28.10 ± 9.39GSNO 0.43 ± 0.02 18.30 ± 6.29 42.17 ± 18.09GSNO + Hb 0.33 ± 0.09 12.48 ± 1.66 26.04 ± 6.48GSNO(light inactivated)0.33 ± 0.03 9.48 ± 2.42 13.69 ± 3.08P700+ absorbance measurementsResults and DiscussionFigure 1. A typical P700 oxidation curve obtained from a pea leaf disk incubated for 70 min under 150 µmol m-2 s-1 white light in distilled water, following the cycle of illumination indicated by arrows and light sources above the trace.Figure 2. A section of the P700 oxidation curve from Fig. 1 (indicated by the dotted rectangle in Fig. 1) showing AL-, ST- and MT-areas.AcknowledgementReferences

The effect of exogenous NO on PSI photochemistry in intact pea leaves

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The effect of exogenous NO on PSI photochemistry in intact pea leaves

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