In this work the biological tank of a WRRF in Italy was monitored placing five floating hoods on a plug-flow-like biological aerated tank surface in order to capture emission dynamics in both time and space domains. The five hoods report which location is more responsible for N2O production at a certain moment of the day. Moreover, with this experimental investigation, a spatial shift in N2O production towards the end of the biological tank could be detected. This provides important insights in the changes in biological dynamics especially with varying incoming load