Maleki Shahraki, Azadeh

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t/,y,"('Fi,y\n j,,,)'fi-u--str {40)01"-5 .,iL.;s .,*"t.r+ c.rtr-ri I '#t,;s[, .6L::t,G.s-is lsb r 6jL3.1ts o't*.;ls,5jLg.;lr ,ef cSl-f t a'.U 0t+t,:b|g:-ei o ;tSlt1.1{o o.;L,ar ;lJ.^Sl ,-r"i s.rit-Fl.i li oljl drtPluntago ovuta * fU ! .jFt .F -i>t -,r":L.i; J1l.rflL" .sl;i:tn;al, 0lrb"lG\*t-*.f 'LlPraA;r."= .t**o ,5sG\* )u* -f ',tY)J3{ +jlr ,rr1f1:+.U 0t"t, .11*., \f11 iLJ-- .Kerman University of Medical SciencesSchool of PharmacyFor the certificate of Pharm. D DegreeTitle: Study of antibacterial and antioxidant activities of hydroalcoholicand aqueous extract of Plantago ovataBy: Azade Maleki shahrakiSupervisors:Dr. Mitra MehrabaniDr. Mohamad Hasan MoshafiDr. Mehrnaz MehrabaniThesis NO:936 Autumn 2017,r-r[! a,-)li:oJ5->,r"t jU \$-pl ajb oL.f,/5J131.r.r o1@ ,itri.Jl ,-r"i t./.t-jSl.t o;i olll d)J.Plantago ovatao)re-l f U q oS c*l Plantaginaceae .:19iE dssl> ff ,_f s, jl ,j! Plantago ovata Forsk :a".ti.L .>;1: ,_? \-b ;: ulljl; qgG orUil +i1r c-y 3 +Jlr "l,L- .tJ ."-1 G-J)l) pl-r.ll .-r.lp ",, a:;[! *JU" ;_1 t<r-,k* jl crJL--i ..1)l ,11 jsL ii--Jgz _,,_l 141; .3 G; r -\r-o; ;: ilrJl .r.l j lL-F! ;l _Ll_"-ojl-*l ,-LGi _*i t o+_,,.ri ft+l ,)f-l oLJ o-;ir ,-s.\ : JyV ,slao 6151. L; ) G\r:-St ;1 c-*-L- o.st r*.-i J;1-l;'-L ,-rss st t "f< +J1r .rJ-il3;r-a s u1i ,;loo,g;;yi ^.2 ;1 -r- :iL;i ;!il "frty o;Las fu "F s:*-t-f -q li.rJJ,rLl ;r o;las js -,^ Jj-r-r)\, j\p s +tf 61;"-' +lJr; J;S Jl4. c-irl; } lo .. DPPH ui)) u..r,l+Jl ;i c-".t-- .-H .fr ,_r-:t..-Qti -u-l ,-Li.J; G.S "rlJl ,5r5L ,-,-JF, c--:a Gs-s J -,6i ,t $: Gs: t s-,)l kV osl-as 5 :l: crL;"; oLJ cl: J;t" o1b .:lUJl il c-JU jl J-.f 5rLl *",ry :6L;.;y; GF! S 6- tr .:3, +lJ;* ;ts-t 4: c-; i ,.j;o ,JU*JI .-r.i c^"-t>- .5lrl> sJ*lr-l., .-r-"-+5 ry (Arng/ml) ALsJ* t:-t )l -;"s (L rng/ml)r,--Jt":*l SJsJ$b-l crS\61, it*\-ue i_y. -*l g;1; o)re-l+lJJ,- osl","s ! crJ aJ*lfL o;Las 1..S3,l Jt-F!;i;l .-u,o>,tL:-mglml) JL*(" 6-U dlJJ* s ,t3,-\r-G ;tJ) J) -p Ci;sl6JSJ.l+k*l ,Jjsq Gi; jr+^a *-!.'*;;L =*r1,* ))s--i:,Ji _nVyj)t*,.J_5 Fii J*u F", y-*6:l:f* 1 .El;.-L^; oJ-aL:- (rr4LSJ* 3 o;4,1,*L .,"l-.L" :;l^.as o: ,n .f y j ,r--f -f :t : ,:4*y-./JL..ti ,*_f )i >{--jo1.*.1,;lr.Jl e\ ,SF\r);l :.g"rJS .l*i,r-J<Jl A-aYiAbstract:Study of antibacterial and antioxidant activities of methanolic and aqueousextract of Plantago ovatuIntroduction: Plantago ovata Forsk is one of two species of important medicinalplantaginaceae family known as psyllium. Medicinal plant organs, including seedand psyllium seed husk have been used extensively in traditional medicine.According to the undeniable role of antioxidant and antibacterial factors in theprocess of many diseases in the present study, the antioxidant and antibacterialactivity of methanolic and aqueous extract of psyllium seed has been investigated.Method of testing: After collection, aqueous and hydroalcoholic extracts of theplant by two methods of extraction, maceration and soxhlet were extracted. Theflavonoid content of both extracts were estimated based on routine and quercetinand total phenol content of both extracts was calculated based on Gallic acid.Antioxidant activity was measured by DPPH method and minimum inhibitoryconcentration was measured by agar dilution method on eight bacterial strains.Results: The results of antioxidant activity of methanolic extract of seed of plantshowed that the extract obtained from the maceration method had strongerantioxidant activity than Soxhlet method. Antibacterial results of macerationmethod for Staphylococcus epidermidis (a mg/ml) was lowerthan Soxhletmethod(8 mg/ml) which show the stronger antibacterial activity of the maceration extractthan the Soxhlet extract for this strain. Also for Staphylococcus aureus, bothmaceration and soxhlet methods showed similar results (32 mflml). For other sixmicroorganisms including Bacillus subtilis, Pseudomonqs aeruginosa,Klebsiellapneumonia, Escherichia coli, Seruatia marcescens, Micrococcus luteus bothmethanolic extracts of maceration and Soxhlet had no acceptable antibacterialeffect.Keywords : antibactrial, antioxidant activities, pl antago ov ataIl

Study of antibacterial and antioxidant activities of hydroalcoholic and aqueous extract of Plantago ovata

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Study of antibacterial and antioxidant activities of hydroalcoholic and aqueous extract of Plantago ovata

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