Peran Gen Polimorfik Δ Asam Amino Levulinat Dehidratase pada Intoksikasi Pb

Abstract

The role of δ amino levulinic acid dehydratase polymorphism gene in lead intoxication.Background: Three distinct isozymes, designated ALAD 1-1, 1-2, and 2-2 are resulted from the expression of two common alleles, designated ALAD1 and ALAD2. The existence of this polymorphism gene whose product was implicated in the pathogenesis of lead toxicity suggested the potential for a genetically determined differential susceptibility. This study was attempted to investigate the role of ALAD polymorphism gene in lead intoxication showed by children blood lead levels.Methods: The study involved 54 children with 5-10-year-old, coming from Gebang Sari, Sekaran and Moro Demak. Cross-sectional design was adopted in this research. Blood lead level was measured by atomic absorption spectrophotometer. The ALAD polymorphism in exon 4 was determined by polymerase chain reaction (PCR) with restriction fragment length polymorphism, according to the methods described by Schwartz et al. Data were not statistically analyzed due to only two samples were ALAD2.Results: Two samples were ALAD2 (1-2 isozymes) with blood lead levels (BLL) of 24.50 ppb and 91.70 ppb respectivelly. The mean of ALAD2 BLL was about 48.60 ppb higher compared to that of ALAD1BLL.Conclusion: Individuals with ALAD2genotype had blood lead levels higher than those of individuals with the ALAD1 genotype, therefore, ALAD2genotype was much more susceptible to lead