The purpose of this study was to find out the fatigue index between Basketball, Handball and Volleyball Players. Fifteen male students from Bharathiar University Constitution College Arts Science Sivagiri were participant randomly sampled. The age of the participant was ranged from 19 to 25 years. The selected variables of fatigue index were assessed by using RAS test. Ensure the subject sprints maximally through the line each time. After 10 seconds, the next sprint starts from the opposite end of the 35 m track. Repeat this procedure until six sprints are completed. The obtained data were statistically analysed ANOVA was used to find out the significant difference, Tukey HSD test was used as a post-hoc test. An alpha level of 0.05 was used for all tests. The results indicate that there is significant difference between Basketball, Handball and Volleyball Players