Barlattani A.

Caponio V. C. A.

Lo Muzio L.

Mascitti M.

Mauceri R.

Procaccini M.

Rubini C.

Santarelli A.

Togni L.

English

Archivio istituzionale della ricerca - Università di Palermo

269PATHOLOGY AND ORAL MEDICINENON-LASER PHOTOBIOMODULATION AND BENZODIAZEPINES IN BMS MANAGEMENTTateo D., Lilli D., Magrì C., Ventura N.Department of Oral Pathology, School of Dentistry, University of Bari, Bari, ItalyAim: the aim of this study was to compare a photobiomodula-tion device (Bioptron™) used as light therapy and Benzodiaz-epines (BDZ) in burning mouth syndrome (BMS) treatment.Methods: clinical and demographical data of 43 patients with a clinical diagnosis of BMS were randomized in two split groups. Group A was treated with Bioptron™ light therapy, whereas group B was treated with BDZ. Visual Analogue Scale (VAS) was used to assess the therapeutic effects of the given treatments in both groups. Outcomes were recorded at the be-ginning of the treatment (t0) and at the end (tf).Results: reduction of VAS was recorded in both groups with a significant improvement (p < 0.05). In Group A, the VAS de-creased significantly (t0: 7.07: tf: 4.64). Also, in group B, mean VAS decreased significantly from 7.52 to 6.41. This study does not show a statistically significant difference between the two therapies (P = 0.064; p > 0.05).Conclusion: results of the present study showed that both treatment were effective in reducing the patient-perceived pain and discomfort, although Bioptron™ light treatment showed a slightly bigger reduction in VAS points. As Bioptron™ light therapy has no known side-effects, and is a non-invasive pro-cedure, this can allow for management of BMS patients in which customary medicaments with BZD may not be feasible. Further studies may focus on using light therapy and pharma-ceuticals in combination, to try and understand if they may work better together in helping BMS patients.IMMUNOHISTOCHEMICAL DETECTION OF BRAF V600E MUTATION IN AMELOBLASTOMATogni L.1, Mascitti M.1, Rubini C.2, Procaccini M.1, Barlattani A.3, Mauceri R.4, Caponio V.C.A.5, Lo Muzio L.5, Santarelli A.11Department of Clinical Specialistic and Dental Sciences, Marche Polytechnic University, Ancona, Italy2Department of Biomedical Sciences and Public Health, Marche Polytechnic University, Ancona, Italy3Department of Clinical Sciences and Translational Medicine, Tor Vergata University, Rome, Italy4Department of Surgical, Oncological and Oral Sciences (DiChirOnS), University of Palermo, Palermo, Italy5Department of Clinical and Experimental Medicine, University of Foggia, Foggia, ItalyAim: the ameloblastoma is the most frequent odontogenic tu-mor. Despite its benign nature, the local aggressiveness and a high recurrence rate characterize some entities. Literature da-ta suggest the BRAF V600E mutation is involved in 46-82% of ameloblastomas, offering a biological rationale for developing new therapeutic strategies. The study aims to evaluate the correlations between the presence of BRAF V600E mutation and the clinicopathological data in a cohort of ameloblastoma patients.Methods: 50 ameloblastomas were subjected to immunohis-tochemical analysis using VENTANA anti-BRAFV600E Mouse Monoclonal Primary Antibody. A uniform, cytoplasmic staining of viable tumor cells was considered as “positive” staining. Dif-ferences among ameloblastoma groups were established by Chi-square and Mann-Whitney tests. A p-value < 0.05 was ac-cepted as significant.Results: the 46% of ameloblastomas harbored the BRAF V600E mutation. Its presence was significantly associated with the man-dibular site (l = 0.0004) and unicystic histotype (l = 00383). All BRAF unicystic ameloblastomas arose in the mandible (l < 0.0001). There was a trend between the presence of BRAF and the late onset of recurrent (l = 0.0521), without reaching a signifi-cance difference in term of disease-free survival time.Conclusion: our results suggest the MAP-kinase pathway could contribute on ameloblastic tumorigenesis. Moreover, they could indicate the anatomical specificity of the driving mutations of mandibular tumors, providing a biological ration-al for developing new targeted therapies.

IMMUNOHISTOCHEMICAL DETECTION OF BRAF V600E MUTATION IN AMELOBLASTOMA

https://iris.unipa.it/bitstream/10447/515616/2/Mauceri%20-%20IMMUNOHISTOCHEMICAL%20DETECTION%20OF%20BRAF%20V600E%20MUTATION%20IN%20AMELOBLASTOMA.pdf

IMMUNOHISTOCHEMICAL DETECTION OF BRAF V600E MUTATION IN AMELOBLASTOMA

Abstract

Similar works

Full text

Available Versions

Archivio istituzionale della ricerca - Università di Palermo