This study investigates the relationship between spirituality and locus of control using similar definitions and processes of previous research on the topic. This cross comparison of participants who categorize themselves into different groups of spirituality allows for the examination of how people of various spiritual groups can be described as having an internal or external locus of control. Items from questionnaires completed by an undergraduate population were analyzed using a one-way ANOVA to examine the direct relationship between the variables. A significant difference between the groups of participants of differing spiritualities and their locus of control was found. Participants who consider themselves atheists were found to have higher levels of internal locus of control versus participants who labeled themselves agnostic, unsure, spiritual, religious, or a combination of labels. Participants who described themselves as agnostic, unsure, spiritual, religious, or a combination of levels were found to have higher levels of external locus of control versus those who described themselves as atheists. These results offer support for past research and open opportunities for the continuance of studying aspects of the relationship between spirituality and locus of control