The aim of this study is to validate and adapt to the Spanish context of Physical Education (PE) the State Mindfulness Scale (SMS). The study included 1428 students aged 13–19. In order to analyse the psychometric properties of the SMS, an exploratory factorial analysis was carried out to confirm the presence of two factors, a confirmatory factorial analysis that has offered support for both the structure of two factors. We also included an analysis of temporal stability, an analysis of invariance across gender, an analysis of internal consistency and an analysis of predictive validity through a t-test in which mental health (β = −0.28, p < 0.001) and physical or bodily experience (β = −0.16, p < 0.001) negatively predicted anxiety, respectively. The structure of the questionnaire was valid and reliable, gender-neutral and with adequate levels of temporal stability