Angelidaki, Irini

Campanaro, S.

Fontana, A.

Kougias, Panagiotis G.

Treu, Laura

Zhu, Xinyu

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Jan 01, 2018Insights on the activity of the anaerobic digestion microbiome by means ofmetatranscriptomic functional investigationTreu, Laura; Campanaro, S.; Fontana, A.; Zhu, Xinyu; Kougias, Panagiotis G.; Angelidaki, IriniPublished in:Book of Abstracts, Sustain 2017Publication date:2017Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Treu, L., Campanaro, S., Fontana, A., Zhu, X., Kougias, P. G., & Angelidaki, I. (2017). Insights on the activity ofthe anaerobic digestion microbiome by means of metatranscriptomic functional investigation. In Book ofAbstracts, Sustain 2017 [R-18] Technical University of Denmark (DTU).Insights on the activity of the anaerobic digestion microbiome by means of metatranscriptomic functional investigation L. Treu1, S. Campanaro2*, A. Fontana1,3, X. Zhu1, P.G. Kougias1 and I. Angelidaki11: Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark  2: Department of Biology, University of Padova, Via U. Bassi 58/b, 35121 Padova, Italy 3: Department for Sustainable Food Process – DiSTAS, Catholic University of the Sacred Heart, 29122 Piacenza, Italy *Corresponding author email: stefano.campanaro@unipd.itNowadays there is an urgent need of a sustainable solution for energy generation and waste resource recovery. Biogas production from Anaerobic Digestion (AD) of organic matter is an attractive technology that gained increasing attention as renewable  source of energy. Anyway, the intricate set of microbial species that by their activity and abundance drives the AD process is far from being completely understood (Campanaro et al., 2016). In order to produce high-value products, i.e. almost pure methane, the influence of operational parameters (e.g. temperature, pH, feedstock composition, etc.) on microbial activity needs to be addressed. A set of metatranscriptomic studies have been performed in replicate biogas reactors with the aim of correlating the microbial community structure and their changes in gene expression with the variations in process parameters. The first study investigated the transcriptional changes in  a community after radical shifts of the influent feedstock determined by the addition of long chain fatty acids (LCFA). The importance of Syntrophomonas species was confirmed and the activation of chemotaxis genes was evidenced in several species; moreover an increased gene expression in Methanosarcina sp. was recorded (Treu et al., 2016). Other two ongoing studies are focused on hydrogenotrophic methanogenesis and the role of syntrophic bacteria in  the biogas production improvement obtained by the conversion of CO2 into CH4 via external hydrogen addition. Moreover, acetate accumulation and pH fluctuations were analyzed to identify the microbial key functions and correlate them with the disturbances affecting the AD process. Results shed light on the inhibitory conditions that can rise during the process, deciphering some of the metabolic pathways present in the species responsible for acetate production and accumulation. References Campanaro, S. et al. (2016) ‘Metagenomic analysis and functional characterization of the biogas microbiome using high throughput shotgun sequencing and a novel binning strategy’, Biotechnology for Biofuels, 9(1), p. 26. doi: 10.1186/s13068-016-0441-1. Treu, L. et al. (2016) ‘Untangling the Effect of Fatty Acid Addition at Species Level Revealed Different Transcriptional Responses of the Biogas Microbial Community Members’, Environmental Science and Technology. Elsevier, 46(11), pp. 23–32. doi: 10.1021/acs.est.6b00296. Sustain Abstract R-18

Insights on the activity of the anaerobic digestion microbiome by means of metatranscriptomic functional investigation

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Insights on the activity of the anaerobic digestion microbiome by means of metatranscriptomic functional investigation

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