One of the major concerns in the establishment of any mycorrhization
program is ensuring the mass production of sterile, consistent and standardized
plant material. In the present study, a successful protocol for micropropagation of
Cistus salviifolius L. was developed. The process was initiated from nodal segments
excised from mature C. salviifolius plant selected due to its mycorrhizal capacities.
Murashige and Skoog basal medium supplement with gibberellic acid (0.5 mg/L)
and of 6-Benzylaminopurine (0.5 mg/L) was the best medium for proliferation
purposes and successful rooting was achieved with the same basal medium
supplemented with Indole-3-butyric acid (0.5 mg/L). The proposed methodology
represent a novelty because it allowed the rapid multiplication of C. salviifolius
starting from mature explants, here reported for the first time, using lower plant
growth regulators concentrations than the previously reported for this particular
Cistus specie