Background and aim: Delay in diagnosis of breast cancer is still a main cause of death among disease women. However, preventive procedures to solve this problem are less than expectation. The success rate in the treatment of breast can be directly related to early diagnose and breast self –examination is one of the ways to detect the disease early. This study was aimed to examine the effect of education on knowledge, attitude and performance of employed women about breast self-examination. Methods: In this quasi-experimental study, 50 employee women randomly selected of Shahrekord University randomly. Participants during 2 weeks, in 2 sections and each session 2 hours performed self –examination. The rate of knowledge, attitude, and performance of participants before and after education using a questionnaires and check list were evaluated and compared. Results: Based on the results, the mean scores of subjects from 33.5±18.4 to 68.9± 9/2 increased (p<0.001). The mean scores for attitude increased from 74.5± 14.7 to 82.2±10.2 and the mean score for function from 41.5±30.8 before training to 84.8± 7.8 after training (p<0.001). Before training, %30 and after training, %84 of the wards under research breast self-examination performed. BSE McNamar test showed a significant difference before and after training breast self-examination (p<0.001). Conclusion: According to the present study, educational programs designed to enhance the knowledge, attitude and function and representation it through social media and health care centers is commend to provide opportunities for the use of screening methods