Background: There is a worldwide interest in searching for potential and effective medicinal plant candidates against various diseases or disorders. Till now, there are number of anti-inflammatory drugs like NSAIDs are available which showed various adverse effects in our body. To reduce these adverse effects, some of medicinal plants have been experimentally validated. Aim: The aim of our immunopharmacological study is to investigate the immunosuppressive and anti-inflammatory activity of aqueous leaves extract of Mangifera indica in human peripheral blood mononuclear cells (PBMC) extracellular against hepatitis B vaccine antigen (HBsAg) Methods: In this study, aqueous leaves extract were collected from Mangifera indica and determined its effect on total blood counts (using forward and side scatter) and monocyte CD14 FITC surface markers using flow cytometry. In addition, the effect of aqueous leaves extract on nitric oxide (NO) production from PBMC cell culture supernatant and also estimate its proliferation assay using Concanavalin A (Con A, 0.5 mg/ml, 50 µl). Data analysis was performed using BD cell Quest Pro software for flow cytometric analysis and one way ANOVA test (Boniferroni multiple comparison test). Results: The results displayed that aqueous leaves extract of Mangifera indica showed dose dependent decline in blood counts (increased in side scatter and slightly decreased in forward scatter), monocyte CD14 FITC surface marker; Con A proliferation and nitric oxide production from cell culture supernatant in human PBMC. Conclusion: Overall, the results claimed that aqueous leaves extract of Mangifera indica at higher doses showed immunosuppressive and anti-inflammatory activity
