Amini, Asadollah.

Ghatreh Samani, Keihan.

Jafari, Laleh.

Koohi Habibi Dehkordi, Arash.

shahrekord university of medical scinces

Poster – [A-10-624-1]The comparison of net palem olein, hydrogenized+palem andcanola oil on dyslipidemiaJafari Laleha, Amini Asadollahb,Ghatreh Samani Keyhanb, Koohi Habibi Dehkordi Arashaa34 N, Zafar St, Saadi Gharbi St, Shahrekord, IranbShahrekord University of Medical Sciences, IranE-mail addresses: jafarilaleh@yahoo.com (J. Laleh),SAAMINI@yahoo.com (A. Asadollah),kgsamani@yahoo.com (G.S. Keyhan),Arash_koohi_ar@yahoo.com (K.H.D. Arash)Introduction: Palem olein is structured from meaty part of palemculture. Recent investigation showed that this oil is safe for healthbecause consumption of palem olein not only decreases LDL but alsoincreases HDL and prevention of hypertension by prevention ofoxidative stress. In contrast other intakable oil such as hydrogenizedoil may led to high bad cholesterol in human body. The aim of thisstudy was to understand the effect of palem olein on blood fat in ananimal model.Material and methods: This is an experimental study that wasconducted in Medical University of Shahrekord in 1389. Eighty eightWistar rats with the same weight were provided and primary care inoptimal environment for 2 weeks. Then theywere randomly divided inthe following 4 groups: a) control with normal diet, b) hydrogenizedoil+palem+normal diet, c) net palem olein+normal diet, andd) canola oil+normal diet. All samples were feed and observed for4 weeks and total cholesterol, triglyceride, HDL and LDL weremeasured at the end of intervention. The data after collection wereanalyzed by SPSS software and application of ANOVA test.Results: The difference of every one of fat profiles mean totalcholesterol, TG, LDL, HDL and LDL/HDL between four groups wasstatistically significant (P<0.001).Conclusion: According to results of this study and comparison ofresults with other studies although palem olein probably could reducebad cholesterol and triglyceride but other risk factors such as diet andgenetic characteristics that influence on fat level must be noticed.Keywords: Palem olein, Canola oil, Hydrogenized oil, HDL, LDL,Cholesteroldoi:10.1016/j.clinbiochem.2011.08.552Poster – [A-10-678-1]Non enzymatic antioxidant status in the users of pan masalacontaining tobacco (PMT)Kumar Shrestha Raj, Das BinodKumar Lal, Mehata Kisundeo,Jha Pankaj, Baral Nirmal, Lamsal MadhabDepartment of Biochemistry, Dharan, NepalE–mail addresses: raj@kusms.ku.edu.com (K.S. Raj),raj.shrestha2@yahoo.com (M. Kisundeo),gotorose@gmail.com (J. Pankaj), nirmalbaral@yahoo.com (B. Nirmal),madhablamsal@yahoo.co.uk (L. Madhab)Background: The use of pan-masala containing tobacco (PMT) isincreasing rapidly in countries like Nepal and India. It is a health hazarddue to the presence of nitrosamineswhich lead to production of reactiveoxygen species (ROS), thereby disturbing the oxidant/anti-oxidantbalance in the body. The present study aims to find out the oxidativestress and antioxidant status in PMT users.Materials and methods: A cross-sectional study was carried out byusing snowball sampling technique. 50 healthy PMT users and 53healthy individuals (non-PMTuserswere enrolled). Blood sampleswerecollected in EDTAandplain vacutainers. VitaminC, vitamin E, and serumMDA were estimated using standard method, while uric acid, totalbilirubin and albumin were estimated using diagnostic reagents fromRFCL, India. The data were analysed by using Student's ‘t’ test.Results: The results showed that in PMT users, there was asignificant (p<0.05) fall in plasma antioxidant parameters; vitamin Clevel (1.207±0.734), vitamin E (0.791±0.232), albumin (3.75±0.701)as compared to the non PM-T user subjects and fall in uric acid level(4.11±1.61) were not significant (p=0.756) as compared to non-PMTuser subjects (4.19±1.12). MDA level was significantly increased(p<0.001) in PM-T users (10.78±9.08) as compared to PMT nonusers (4.58±4.36).Conclusion: MDA level was significantly increased in PMT users,that indicates lipid peroxidation and most of the non enzymaticantioxidant parameters were decreased so PMT users are at high risk offree radical damage which may lead to malignant transformation,cancer, CHD and others.Keywords: ROS, Oxidative stress, Antioxidant, PMTdoi:10.1016/j.clinbiochem.2011.08.553Poster – [A-10-685-1]Investigation of local probiotic bacteria as live sublingual vaccinesfor treatment of atopic disease in vivoBasirnezhad Masouda, Roozbeh Leilab, Varasteh Abdol-rezaaaBu-Ali Research Institute Ferdossi Square, Bu-Ali Square Mashhad, IranbIslamic Azad University, Nour Branch, IranE-mail addresses: Basirnezhad.English@gmail.com (B. Masoud),leila_roozbeh@yahoo.com (R. Leila),varastehA@mums.ac.ir (V. Abdol-reza)Introduction: Probiotics are defined as live microorganisms that,when administered in adequate amounts, confer a health benefit onthe host. Recently probiotics may serve as both attractive adjuvantsystems for improved allergy treatment and as a new treatmentapproach that uses selected probiotic strains as mucosal antigendelivery vehicles for recombinant allergens.Materials and methods: 6–8 weeks old inbred female BALB/cwere immunized intraperitoneally with recombinant chenopodiumalbum 2 (rche a 2) followed by aerosol challenge of 1% rche a 2.Sensitized mice were classified in 8 groups of six mice and thenindividual groups were treated sublingually with individual orcombination of different probiotic bacteria (Lactobacillus plantarumand Bifidobacterium bifidum). After undergoing immunotherapy,blood samples were collected from all mice for IgE assays. Spleenswere also excised and cells were cultured in complete RPMI mediumthen cell culture supernatants used for cytokine assays.Results: Sensitization with rche a 2 induced high levels of IgE. Wedo expect elevation of Th1 cytokines profile and Tregs in groupswhich probiotics are used and reduction in Th2 cytokines profile.Conclusion: Probiotic products are recommended to be adminis-tered in combinations, as these may be functionally more efficientthan individual strains. They may reinforce Th1 type immuneresponses and suppress Th2 cytokines. In particular, dietary probio-tics offer the potential for a cheap and safe prevention strategyagainst allergic disease.Keywords: Probiotics, Sublingual, Atopic, Lactobacillus,Immunotherapydoi:10.1016/j.clinbiochem.2011.08.554Abstracts S229

The comparison of net palem olein, hydrogenized plus palem and canola oil on dyslipidemia

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