Imani, Reza.

Moazeni Bistgani, Mohammad.

Iranian Red Crescent Medical Journal

PubMed

shahrekord university of medical scinces

Iranian Red Crescent Medical Journal. 2013 August; 15(8): 759-61. DOI: 10.5812/ircmj.3883Published Online 2013 August 05. LetterBacteria Isolated From Patients With Cholelithiasis and Their Antibacterial Susceptibility PatternMohammad Moazeni Bistgani 1, *, Reza Imani 21 Department of Surgery, Faculty of Medicine, Shahrekord University of Medical Sciences Shahrekord, IR Iran2 Department of Infectious Diseases, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, IR Iran*Corresponding author: Mohammad Moazeni Bistgani, Department of Surgery, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, IR Iran. Tel: +98-9131812974, Fax: +98-3812224492, E-mail: dr_m_moazeni@yahoo.com. Received: December 17, 2011; Revised: November 23, 2012; Accepted: January 08, 2013Keywords: Antibacterial Susceptibility Pattern; Bacteria; CholelithiasisImplication for health policy/practice/research/medical education:Determine the exact incidence and nature of the microbial flora in bile of the patients with cholelithiasis and chronic cholecystitis as well as their anti-biotic sensitivity pattern.Copyright © 2013, Iranian Red Crescent Medical Journal; Licensee Kowsar Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Dear Editor,The most prevalent complication of cholelithiasis is chronic cholecystitis usually necessitating cholecystec-tomy (1). The biliary tract is usually sterile; however, if cholelithiasis is pigmented and cholestering, different microbes might be identified in and/or cultured from the bile or gallbladder wall (2). Microscopic examina-tions indicated that 20-50% of the patients with chronic cholecystitis have positive bile culture (3). Different rea-sons for biliary tract infection have been presented, e.g. ascending infection due to reflux of duodenal contents, blood-borne infection and infection spread through the portal-venues channels. Ascending infection from the duodenum is thought to be the primary mechanism by which bacteria enter the bile (4). Different microbes in the bile may be cause to post- cholecystectomy infec-tions. Thus, understanding the most common organisms causing them and their antibacterial susceptibility pat-tern would be useful in prevention of these infections. The present study was carried out to achieve this aim.In this cross sectional study, 132 patients with cho-lelithiasis and chronic cholecystitis without risk fac-tors for postoperative sepsis were subjected to elective laparoscopic cholecystectomy from September 2009 to September 2010. We ordered antibiotic prophylaxy rou-tinely with 1gr keflin one house before operation. Using a questionnaire, patients' age, sex, clinical features of the patients, the isolated bacteria, and antibiogeram were re-corded. During surgery 5-10 ml of the bile samples were aspirated with sterile syringe from gallbladder immedi-ately after cholecystectomy; 3-5 ml was placed in a sterile container; another 3-5 ml was inoculated directly into an aerobic and anaerobic BacT/Alert blood culture bottle. The Samples were sent immediately to the Laboratory and incubated at 37°C for 24h. After the bacteria were iso-lated, we performed antibiotic sensitivity tests by the iso-lates carried out using Kirby-Bauer method with a colony in Mueller-Hinton agar medium (pH 7.2-7.4). The results were reported as susceptible, resistant and intermedi-ate based on the diameter of the clear zone around disks with reference to the antibiotic standard table. The Data were analyzed by Chi-Square test and P<0.05 was consid-ered significant.From the total 132 bile samples, bacteria were isolated in 50 samples (37.87%). Some other studies have shown that 20-50% of the patients with chronic cholecystitis have positive bile culture (3). Mean age of patients with positive and negative cultures was 56.9 ± 14 and 52.7 ± 14.7 years respectively, having significant difference based on t student test (P < 0.05). A study by Al Harbi et al. has shown being older than 50 years was the only factor sig-nificant in view of preoperative positive bile culture (5), which is in agreement with our study. Anaerobic bacteria were detected in 8 (16%), Monomicrobial infection in 47 (94%), and Polymicrobial infection in 3 (6%) patients. In a study by Ballal et al. in India, bile cultures for aerobic and anaerobic bacteria were carried out on 125 samples from patients with chronic cholecystitis with cholelithiasis; 71 (56.8%) aerobic and 17 (13.6%) anaerobic bacteria were detected. Among the mixed flora, 2 had only aerobes and the remaining 5 had both aerobes and anaerobes (6). Al Harbi et al.'s study also showed Polymicrobial infection in 4 (3.57%) and anaerobic bacteria in none of the cases (5). Anaerobic bacteria may grow when the bile duct is seriously infected by anaerobic and aerobic bacteria and hence body's immunity becomes low. Lou et al. suggested Moazeni Bistgani M et al.Iran Red Crescent Med J. 2013;15(8)760the paucity of anaerobes in the human biliary system (7). The effect of anaerobic bacteria on bile pigment stone has been reported widely (8), according to which the an-aerobes such as B. fragilis and B. fusiformis may produce an E. coli substance called β-Lactamase which resolves bil-irubin. The bilirubin when integrated with calcium ion forms calcium bilirubinate.The difference in anaerobic and Polymicrobial positive culture between our study and others' could be attribut-ed to the method of antibiotic therapy; here, we ordered antibiotic prophylaxy routinely while in Ballal et al.'s study it was selectively and in Al Harbi et al.'s study we had not known.In our study E. coli was the most common isolate (13; 26%), as previously reported (9). Enterobacter was the second one (9; 18%) followed by Salmonella Typhi (7; 14%), Coagolase-negative staphylococcus (6; 12%), Klebsi-ella pneumoniae (2; 4%) and Proteus (2; 4%). The signifi-cance of E. coli dominance is also supported by Maki's studies (1966) indicating a potential role for E. coli's Glu-cosonidase enzymatic activity in formation of calcium bilirubinate gall stone (10). In our study, Salmonella Typhi grew in 18% cases, sensitive to clindamicin and novobiocine in 88.8%. The prevalence of Salmonella Ty-phi in bile of cholelithiasis patients varied widely from 1% to 34% (11), perhaps due to typhoid fever which is, similar to some parts of our country, endemic in some regions. Sexwise analysis of our patients showed that Salmonella Typhi, more common in females compared to males, was isolated only from samples of female pa-tients, reported elsewhere. Its high incidence in females has been attributed to hormonal effects related to men-strual cycle and pregnancy (6). Eslami et al.'s work con-ducted in Iran indicated E. coli (25%) as the most preva-lent isolated bacterium, and the prevalence of Klebsiella, Aerobacter, Pseudomonas, Enterococci, and Proteus as 12%, 10%, 9%, 8%, and 3% respectively (12). In Ballal et al.'s study E.coli and Klebsiella were predominant among the aer-obes with 45.07% and 25.35% prevalence respectively and B. fragilis (58.82%) predominant among the anaerobes (6). In the study of Al Harbi et al. the most common or-ganisms isolated were E. coli (26.1%), Enterococcus facials (15.6%), and Pseudomonas aeroginosa (9.6%) (5). we don’t know why our findings were not completely similar to others'. However, we support taking cultures of the bile at cholecystectomy because appropriate antibiotics can be administered in case of cultures being positive, hence avoiding serious complications, e.g. gram nega-tive septicemia. In our study E. coli was 92.3% susceptible to Amikacin and Enterococci 69.23% susceptible to Ce-phtriaxone. The most susceptible antibiotic for micro-organisms on the whole was Amikacin. Ballal et al. have shown anaerobes were sensitive to Cefotaxime, Metro-nidazole, Chloramphenicol, Cefazolin, and tetracycline, and aerobes isolated to Ampicillin, Chloramphenicol, streptomycin, tetracycline, Gentamicin, and second generation Fluoroquinolones such as ciprofloxacin and Norfloxacin (6).In spite of the fact that we ordered antibiotic prophy-laxy for all of the patients, bacterial isolates and infec-tious complications showed no significant difference. Thus, we recommend starting antibiotics selectively, if supported by the clinical conditions and/or culture re-ports, in case of cholecystitis and cholelithiasis. Howev-er, routine culture of all bile samples is strongly advised.Given our and others' studies anaerobes are rare in the human biliary system; therefore, if antibiotic therapy is considered, aerobic coverage should be satisfactory. In addition because the most susceptible antibiotic for microorganisms on the whole was Amikacin, this anti-biotic can be started selectively.AcknowledgementsThe authors would like to thank Research and Technol-ogy Department of Shahrekord University of Medical Sci-ences for cooperating in this study and Kashani Univer-sity Hospital staff for assisting in data collection.Authors’ ContributionNone declared.Financial DisclosureNone declared.Funding/supportNone declared.References1.       Vitetta L, Best SP, Sali A. Single and multiple cholesterol gallstones and the influence of bacteria. Med Hypotheses. 2000;55(6):502-6.2.       Monstein HJ, Jonsson Y, Zdolsek J, Svanvik J. Identification of Helicobacter pylori DNA in human cholesterol gallstones. Scand J Gastroenterol. 2002;37(1):112-9.3.       Martin LF, Zinner SH, Kagan JP, Zametkin AJ, Garrity FL, Fry DE. Bacteriology of the human gallbladder in cholelithiasis and cholecystitis. Am Surg. 1983;49(3):151-4.4.       Fukunaga FH. Gallbladder bacteriology, histology, and gall-stones. Study of unselected cholecystectomy specimens in Ho-nolulu. Arch Surg. 1973;106(2):169-71.5.       Al Harbi M, Osoba AO, Mowallad A, Al-Ahmadi K. Tract micro-flora in Saudi patients with cholelithiasis. Trop Med Int Health. 2001;6(7):570-4.6.       Ballal M, Jyothi KN, Antony B, Arun C, Prabhu T, Shivananda PG. Bacteriological spectrum of cholecystitis and its antibiogram. Indian J Med Microbiol. 2001;19(4):212-4.7.       Lou MA, Mandal AK, Alexander JL, Thadepalli H. Bacteriol-ogy of the human biliary tract and the duodenum. Arch Surg. 1977;112(8):965-7.8.       Leung JW, Liu YL, Lau GC, Chan RC, Lai AC, Ling TK, et al. Bacte-riologic analyses of bile and brown pigment stones in patients with acute cholangitis. Gastrointest Endosc. 2001;54(3):340-5.9.       Cheslyn-Curtis S, Russell RC. New trends in gallstone manage-ment. Br J Surg. 1991;78(2):143-9.Moazeni Bistgani M et al.761Iran Red Crescent Med J. 2013;15(8)10.       Nagase Masao, Tanimura Hiroshi, Setoyama Motoichi, Hikasa Yorinori. Present features of gallstones in Japan: A collec-tive review of 2,144 cases. The American Journal of Surgery. 1978;135(6):788-790.11.       Sattar I, Aziz A, Rasul S, Mehmood Z, Khan A. Frequency of infec-tion in cholelithiasis. J Coll Physicians Surg Pak. 2007;17(1):48-50.12.       Eslami Gita, Nowruzi Jamileh, Fllah Fatemeh, Goudarzi Hosse-in, Hakemivala Mojdeh, Jahangiri Sahar. Detection of bacteria responsible for gallbladder inflammation and gallstones. Iran J Clin Infect Dis. 2007;2(3).

Bacteria Isolated From Patients With Cholelithiasis and Their Antibacterial Susceptibility Pattern

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