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Magnetic field effects on seed germination and activities of some enzymes in cumin

Abstract

Failure of germination among Apiaceae family seeds, e. g. cumin (Cuminum cyminum), has been reported by many investigators. Until now few works have been done on seed germination and seedling growth of cumin. In this research the effects of the exposure of cumin seeds to magnetic fields on seed germination, early growth, and enzyme activity have been studied under laboratory conditions. Seeds were magnetically exposed to one of three magnetic field strengths, 25, 50 and 75 mT for different periods of time (15, 30, and 60 min). The germination test was performed according to the guidelines issued by the International Seed Testing Association. Enzymes related to the germination process in magnetically exposed and unexposed germinating cumin seeds were assayed after 24 hours of imbibitions in distilled water. Exposure of cumin seeds to different intensities of magnetic fields prior to germination significantly increased germination-related characters. The increase in germination, speed of germination, shoot length, root length, total seedling length, seedling fresh weight, and seedling dry weight was, respectively, 14-17%, 14-57%, 8-27%, 25-62%, 16-39%, 10-29%, and 17-49% compared to untreated control seeds. The calculated vigor indices I and II also increased by 33-73% and 38-72%, respectively. In germinating seeds, enzyme activities of alpha-amylase, dehydrogenase, and protease were significantly higher in treated seeds in contrast to controls and the maximum value was 50 mT for 60 min exposures. The higher enzyme activity in magnetic-field-treated cumin seeds could trigger fast germination and early vigor of seedlings

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