Background: Since there is relatively little information pertaining to the effect of hydroalcoholic extract of Salvia officinalis on the reproductive system of animals, this study was carried out to examine the effect of hydroalcoholic leaf extract of Salvia officinalis on serum levels of LH, FSH, testosterone and testicular tissue in male rats. Materials and Methods: In this experimental study, 31 male Wistar rats were randomly allocated into five groups: the control, normal saline and Salvia officinalis extract (100, 150 and 200 mg/kg/body weight) receiving groups. Rats were intraperitoneally injected with Salvia officinalis extract once daily (at 10 a.m.) for 30 days. Blood samples were collected using a cardiac puncture method and the hormone levels were measured using the radioimmunoassay. Moreover, testes were removed and after fixation, sections were cut and stained for histological studies. Results: The results indicated that Salvia officinalis extract (150 and 200 mg/kg) increased the serum testosterone level (