Evaluation of 11-hydroxy-8-THC-dimethylheptyl effects on cytokines profile and locomotor tests in experimental autoimmune encephalomyelitis

Abstract

One of the components that activate cannabinoids (CB) receptors is 11-hydroxy-�8-THC-dimethylheptyl(HU-210). The present study was conducted to evaluate the effectiveness of HU-210 treatment tocytokines profile and locomotor tests in the experimental autoimmune encephalomyelitis (EAE) modelin the mice. A total of 48 male C57BL/6 mice were placed in 8 therapeutic groups. Mice were immunizedwith myelin oligodendrocyte glycoprotein (MOG) 35-55. When EAE was observe� HU-210 wassubcutaneously administered in 3 groups with different doses: 3,10,30 mg/kg. Control groups weretreated to vehicle or HU-210. Drug or vehicle was given once on the following days: 1, 3, 5, 7, 9, 11, 13,15, 17. During the study clinical scores of disease and balance beam test were analyzed. After 17 daysof treatment, the animals sacrificed and, TNFα, IL-12, IL-4 level in serum was measured using enzymelinkedimmunosorbent assay (ELISA). Our results showed that HU-210-treated mice had significantlyless clinical score of EAE than non-treated EAE induced mice (p<0.05). Data revealed that dose 30mg/kg HU-210 in EAE induced mice significantly decreased serum TNFα, IL-12 (p<0.05). Also, Serum IL-4 levels increased significantly in mice receiving 3, 10, 30 mg/kg dose of HU-210 (p<0.05). Present studyrevealed the beneficial effects of HU-210 in the model of EAE, and suggests its potential use as a drugfor the treatment of multiple sclerosis (MS). © 2011 Academic Journals