Inhibitory effect of tannic acid and its derivative (gallic acid) against cisplatin–induced thiobarbituric acid reactive substances(TBARS)production in rat kidney –in vitro

Abstract

Increasing amounts of evidence suggests tannic acid (TA) and its derivative, gallic acid (GA) has beneficial effects against nephrotoxicity. The aim of this study was to investigate the effectof TA and GA (two structurally related phenolic acids)against cisplatin-induced thiobarbituric acid reactive substances(TBARS) production in rat kidney in vitro. The effect of both phenolic acids on kidney TBARS level in the presence of cisplatin, an anti-neoplastic drug used in the treatment of many solid-tissue cancers which has its chief side effect in nephrotoxicity, was assessed. Thereafter, the antioxidant properties were also determined. Incubation of the kidney tissue homogenate in the presence of cisplatin (1 mM) caused a significant (P<0.05) increase in the TBARS level. However, both phenolic acids caused a significant (P<0.05) decrease in the TBARS content of the kidney in a dose dependent manner (0 –12.5 μM). Nevertheless, TA had a significantly higher (P<0.05) inhibitory effect on TBARS production than gallic acid judging by their IC50value.Furthermore, TA possesses stronger antioxidant properties than GA as revealed by their scavenging, chelating and reducing ability.The inhibition of cisplatin–induced thiobarbituric acid reactive substances(TBARS)production in rat kidney by both TA and GA could be due to their antioxidant properties and this provide further insight into the mechanism of action for their nephroprotective properties from previous reported experimental studies.However, the study revealed that hydrolysis of TA reduces its potenc