Organic farmers are interested in adopting conservation tillage to preserve soil quality and fertility and to prevent soil erosion. Within the framework of a French national study, we compared conventional (ploughing) and conservation tillage systems in organic farming for arable and vegetable cropping systems. Field experiments and on-farm surveys were conducted in several regions of France in order to assess the effects of different tillage systems on soil fertility (physical, chemical, biological) and on weed and crop development. Conservation tillage techniques induced a more compact soil, an increase of carbon and microorganisms in the first soil layer, and an increase of earthworm biomass for very superficial tillage. Weed control was only a major problem for the very superficial tillage, which in turn generated lower crop yields than conventional tillage. The main issues raised by this programme deal with the long-term effects of these techniques on soil fertility, and the improvement of conservation tillage techniques in organic farming