Biomimetics are a growing field with several applications in Bioengineering. This work focuses on two examples of biomimetic systems applied to the fields of Biocatalysis and Biosensing. Enzymes are highly versatile catalysts present in biological systems with a coveted technological potential. Enzymes tend to be large and complex proteins and the development of simpler Biomimetic catalysts has been a long-term goal. In this work, two computationally designed protein-based peptides, RD01v2 and RD02, mimicking metalloprotease activity, were synthesized by SPPS and purified by Preparative HPLC Chromatography. The characterization process was divided in folding studies by far-UV CD spectroscopy and hydrolytic activity studies. All the assays were accomplished for a range of pH from 7 to 10, with and without added Zinc (II) at constant temperature. A Zinc titration at all these pH values, with the mentioned conditions, was performed to calculate the apparent dissociation constant (KpepZn,App in the range of 105 M-1). RD peptides presented low catalytic hydrolytic activity towards 4-nPA with second order rate constant (k2 <1 M-1s-1).
The second exampled studied included the development of stimuli responsive materials mimicking the sense of olfaction
