Viral vectors are playing an increasingly important role in the vaccine and gene
therapy elds. The broad spectrum of potential applications, together with
expanding medical markets, drives the e orts to improve the production processes
for viral vaccines and viral vectors. Developing countries, in particular, are
becoming the main vaccine market. It is thus critical to decrease the cost per
dose, which is only achievable by improving the production process. In particular
advances in the upstream processing have substantially increased bioreactor yields,
shifting the bioprocess bottlenecks towards the downstream processing. The
work presented in this thesis aimed to develop new processes for adenoviruses
puri cation. The use of state-of-the-art technology combined with innovative
continuous processes contributed to build robust and cost-e ective strategies for
puri cation of complex biopharmaceuticals.(...
