Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica
Abstract
Dissertation presented to obtain a Ph.D. degree in Sciences of
Engineering and Technology, Cell Technology, at the Instituto de
Tecnologia Química e Biológica, Universidade Nova de LisboaTechnologies enabling expression of recombinant genes in
mammalian cells belong to the tools of the trade in molecular biology.
These technologies can serve a wide variety of purposes, ranging from
basic research to elucidate gene functions e.g., in transgenic mouse
models, to biotechnological applications such as manufacturing
antibodies or producing viral vectors.
In cell line development, the integration of transgenes into the
chromosomal DNA of the host cell is a crucial step, since chromosomal
surroundings have a major impact on the expression of the transgene.
With regard to safety, product consistency and operational transparency,
expression of biotechnological relevant products from single copy DNA
integrants is preferable over a scenario of multi‐gene integrants.(...)I would like to acknowledge the financial support from Fundação para a
Ciência e Tecnologia (Grant SFRH / BD / 22081 / 2005) and Clinigene‐
European Network for the advancement of Clinical Gene Transfer and
Therapy
